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CD24 Antibody

Protein A Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - CD24 Antibody AP50187
    Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-CD24 Polyclonal Antibody, Unconjugated (AP50187) at 1:200 followed by conjugation to the secondary antibody
  • WB - CD24 Antibody AP50187
    Western blot analysis of lysates from Raji,Ramos cell line (from left to right),using CD24 Antibody(AP50187). AP50187 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • IHC - CD24 Antibody AP50187
    Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD24 Polyclonal Antibody, Unconjugated(AP50187) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q07490
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=8; M=8;R=8;35-45(actual) KDa
Antigen Region 27-53aa/76aa
Additional Information
Gene ID 25145
Other Names Signal transducer CD24, Heat-stable antigen, HSA, Nectadrin, CD24, Cd24, Cd24a
Dilution IHC-P~~1:100~1:500
WB~~ 1:1000
IHC~~ 1:200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Cd24
Synonyms Cd24a
Function May have a pivotal role in cell differentiation. The triggering mechanism of signal transduction may be due to the interactions of differentiating cells with the matrix substrate via the carbohydrate structure of the molecule. In this way, the signal transducer can play very different roles in different cell types as a direct consequence of its glycosylation.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Expressed in the central nervous system, in postmitotic cells of spinal cord, hindbrain, midbrain and forebrain. Expressed in epithelium during the development of non- neural tissues. Expressed in tooth development, specifically in mesenchymal cells differentiating into odontoblast in dental papilla, as well as in the developing eye and hair follicle
Citations (0)

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Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.


Kay R.,et al.J. Immunol. 147:1412-1416(1991).
Jackson D.,et al.Cancer Res. 52:5264-5270(1992).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Hough M.R.,et al.Genomics 22:154-161(1994).
Zarn J.A.,et al.Biochem. Biophys. Res. Commun. 225:384-391(1996).

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$ 295.00
Cat# AP50187
(40 western blots)
Availability: 5-7 days
Bulk Size
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