|Application ||WB, FC, E|
|Calculated MW||31683 Da|
|Antigen Region||1-30 aa|
|Other Names||Voltage-gated hydrogen channel 1, Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, HVCN1, VSOP|
|Target/Specificity||This HVCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HVCN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HVCN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.|
|Cellular Location||Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types|
|Tissue Location||Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen|
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Provided below are standard protocols that you may find useful for product applications.
HVCN1 is a voltage-gated proton channel highly expressed in immune tissues. Channels like HVCN1 mediate the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing.
Musset, B., et al. J. Biol. Chem. 285(8):5117-5121(2010)
Lishko, P.V., et al. Cell 140(3):327-337(2010)
Sakata, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2313-2318(2010)
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