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MED8 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MED8 Antibody AP50634
    Western blot analysis of lysates from Hela,HepG2 cell line (from left to right),using MED8 Antibody(AP50634). AP50634 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q96G25
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29,33,19 KDa
Antigen Region 101-129 aa
Additional Information
Gene ID 112950
Other Names Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name MED8
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
Cellular Location Nucleus.
Citations (0)

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Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

References

Brower C.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:10353-10358(2002).
Gregory S.G.,et al.Nature 441:315-321(2006).
Naeaer A.M.,et al.Nature 398:828-832(1999).
Sato S.,et al.J. Biol. Chem. 278:15123-15127(2003).
Sato S.,et al.Mol. Cell 14:685-691(2004).

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$ 295.00
Cat# AP50634
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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