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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Roundabout 4 Antibody (N-term)   

Roundabout 4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Roundabout 4 Antibody (N-term) AP5099a
    Roundabout 4 Antibody (N-term) (Cat. #AP5099a) western blot analysis in 293,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).
  • IHC-P - Roundabout 4 Antibody (N-term) AP5099a
    Roundabout 4 Antibody (N-term) (Cat. #AP5099a) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - Roundabout 4 Antibody (N-term) AP5099a
    Roundabout 4 Antibody (N-term) (Cat. #AP5099a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WZ75
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 107457 Da
Antigen Region 236-264 aa
Additional Information
Gene ID 54538
Other Names Roundabout homolog 4, Magic roundabout, ROBO4
Target/Specificity This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRoundabout 4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ROBO4
Function Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity).
Tissue Location Specifically expressed in endothelial cells. Expressed at sites of angiogenesis in different tumor types
Research Areas
Citations (0)

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Roundabout 4 is receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. This protein may mediate the inhibition of primary endothelial cell migration by Slit proteins.


Jones, C.A., et al. Nat. Cell Biol. 11(11):1325-1331(2009)
Sheldon, H., et al. FASEB J. 23(2):513-522(2009)
Huang, L., et al. Mol. Vis. 15, 1057-1069 (2009)

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$ 295.00
$ 99.00
Cat# AP5099a
(40 western blots)
Availability: In Stock
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