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NR5A1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NR5A1 Antibody (N-term) AP5106A
    Western blot analysis of NR5A1 Antibody (N-term) (Cat#. AP5106a) in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.
  • IHC-P - NR5A1 Antibody (N-term) AP5106A
    NR5A1 Antibody (N-term) (Cat. #AP5106a) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NR5A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - NR5A1 Antibody (N-term) AP5106A
    NR5A1 Antibody (N-term) (Cat. #AP5106a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13285
Other Accession P50569, P79387, P33242, Q04752, Q9GKL2
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51636 Da
Antigen Region 64-93 aa
Additional Information
Gene ID 2516
Other Names Steroidogenic factor 1, SF-1, STF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP, NR5A1, AD4BP, FTZF1, SF1
Target/Specificity This NR5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the N-terminal region of human NR5A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNR5A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR5A1
Synonyms AD4BP, FTZF1, SF1
Function Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO- NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:11479297}
Research Areas
Citations (0)

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NR5A1 is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this protein are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.


Almeida, M.Q., et al. J. Clin. Endocrinol. Metab. 95(3):1458-1462(2010)
Ramayya, M.S., et al. J. Steroid Biochem. Mol. Biol. 119 (1-2), 14-25 (2010)
Catalano, S., et al. J. Biol. Chem. 285(8):5581-5593(2010)

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Cat# AP5106A
(40 western blots)
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