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Histone H1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Histone H1 Antibody AP51255
    Anti-Histone H1 Antibody at 1:1000 dilution + HeLa whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P16401
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 31 KDa
Additional Information
Gene ID 3009
Other Names Histone H15, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1B, H1F5
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HIST1H1B
Synonyms H1F5
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Cellular Location Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin
Tissue Location Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
Citations (0)

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Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

References

Albig W.,et al.Gene 184:141-148(1997).
Marzluff W.F.,et al.Genomics 80:487-498(2002).
Mungall A.J.,et al.Nature 425:805-811(2003).
Ohe Y.,et al.J. Biochem. 106:844-857(1989).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 295.00
Cat# AP51255
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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