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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ATP4B Antibody (N-term)   

ATP4B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATP4B Antibody (N-term) AP5181a
    Western blot analysis of ATP4B Antibody (N-term) (Cat. #AP5181a) in mouse heart tissue lysates (35ug/lane).ATP4B (arrow) was detected using the purified Pab.
  • FC - ATP4B Antibody (N-term) AP5181a
    ATP4B Antibody (N-term) (Cat. #AP5181a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P51164
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33367 Da
Antigen Region 52-78 aa
Additional Information
Gene ID 496
Other Names Potassium-transporting ATPase subunit beta, Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain, ATP4B
Target/Specificity This ATP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human ATP4B.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP4B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP4B
Function Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.
Cellular Location Cell membrane; Single-pass type II membrane protein
Research Areas
Citations (0)

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Background

ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.

References

Bab-Dinitz, E., et al. Biochemistry 48(36):8684-8691(2009) Knouff, C.W., et al. Pharmacogenet. Genomics 18(12):1051-1057(2008) Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)

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Cat# AP5181a
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(40 western blots)
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