ATP4B Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | P51164 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 33367 Da |
Antigen Region | 52-78 aa |
Gene ID | 496 |
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Other Names | Potassium-transporting ATPase subunit beta, Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain, ATP4B |
Target/Specificity | This ATP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human ATP4B. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ATP4B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ATP4B (HGNC:820) |
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Function | The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump (By similarity). Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle (By similarity). |
Cellular Location | Apical cell membrane {ECO:0000250|UniProtKB:P20648}; Single-pass type II membrane protein. Cell membrane {ECO:0000250|UniProtKB:P18597}; Single- pass type II membrane protein. Note=Localized in the apical canalicular membrane of parietal cells {ECO:0000250|UniProtKB:P20648} |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.
References
Bab-Dinitz, E., et al. Biochemistry 48(36):8684-8691(2009) Knouff, C.W., et al. Pharmacogenet. Genomics 18(12):1051-1057(2008) Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
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