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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-Elastin Polyclonal Antibody   

Rabbit Anti-Elastin Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-Elastin Polyclonal Antibody AP52134
    L1 mouse intestine lysates L2 rat lung lysates (30ug) probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (AP52134) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37˚C. Predicted band 66kD. Observed band size: 66kD
    detail
  • IHC-P - Rabbit Anti-Elastin Polyclonal Antibody AP52134
    Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated AP52134 followed by conjugation to the secondary antibody and DAB staining
    detail
  • IHC-P - Rabbit Anti-Elastin Polyclonal Antibody AP52134
    Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated AP52134 at 1:200 followed by conjugation to the secondary antibody and DAB staining.
    detail
  • IHC-P - Rabbit Anti-Elastin Polyclonal Antibody AP52134
    Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated AP52134 at 1:200 followed by conjugation to the secondary antibody and DAB staining.
    detail
  • FC - Rabbit Anti-Elastin Polyclonal Antibody AP52134
    Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody (AP52134) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P15502
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 68398 Da
Additional Information
Gene ID 2006
Other Names WS; WBS; SVAS; Elastin; Tropoelastin; ELN
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
FC~~1:20~1:100
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name ELN
Function Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.
Tissue Location Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin
Citations (0)
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Background

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

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Discontinued
Cat# AP52134
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