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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-N-cadherin Polyclonal Antibody   

Rabbit Anti-N-cadherin Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (AP52149) at 1:300 in 4˚C. Followed by conjugation to secondary antibody at 1:5000 90min in 37˚C.
  • IHC-P - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (AP52149) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
  • IHC-P - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
  • IF - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated secondary antibody used at 1:200 dilution for 40 minutes at 37°C.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF
Primary Accession P19022
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 99809 Da
Additional Information
Gene ID 1000
Other Names CDHN; NCAD; CD325; CDw325; Cadherin-2; Neural cadherin; N-cadherin; CDH2
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
IF~~1:100~1:200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name CDH2
Synonyms CDHN, NCAD
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P15116}; Single-pass type I membrane protein. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes {ECO:0000250|UniProtKB:P15116}
Citations (0)

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Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

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$ 295.00
Cat# AP52149
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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