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>   home   >   Products   >   Primary Antibodies   >   Rabbit Anti-Nanog Polyclonal Antibody   

Rabbit Anti-Nanog Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Rabbit Anti-Nanog Polyclonal Antibody AP52287
    Lane 1: A549 cell lysates probed with Rabbit Anti-Nanog Polyclonal Antibody, Unconjugated (AP52287) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:5000 for 90 min at 37˚C.
  • IHC-P - Rabbit Anti-Nanog Polyclonal Antibody AP52287
    Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (AP52287), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P
Primary Accession Q80Z64
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 34240 Da
Additional Information
Gene ID 71950
Other Names ENK; ecat4; 2412E2Rik; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name Nanog
Synonyms Ecat4, Enk
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15582778}
Tissue Location Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines
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Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

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$ 295.00
Cat# AP52287
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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