|Application ||WB, FC, E|
|Calculated MW||22580 Da|
|Antigen Region||33-60 aa|
|Other Names||Histone H15, Histone H1a, Histone H1b, Histone H1s-3, HIST1H1B, H1F5|
|Target/Specificity||This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human HIST1H1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIST1H1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).|
|Cellular Location||Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin|
|Tissue Location||Ubiquitous. Expressed in the majority of the cell lines tested and in testis.|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
Happel, N., et al. J. Mol. Biol. 386(2):339-350(2009)
Trojer, P., et al. Cell 129(5):915-928(2007)
Wisniewski, J.R., et al. Mol. Cell Proteomics 6(1):72-87(2007)
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