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IL5RA Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IL5RA Antibody (N-term) AP5235a
    Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody (N-term)(Cat. #AP5235a). AP5235a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • IHC-P - IL5RA Antibody (N-term) AP5235a
    IL5RA Antibody (N-term) (Cat. #AP5235a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL5RA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - IL5RA Antibody (N-term) AP5235a
    IL5RA Antibody (N-term) (Cat. #AP5235a) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01344
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47685 Da
Antigen Region 49-76 aa
Additional Information
Gene ID 3568
Other Names Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, CD125, IL5RA, IL5R
Target/Specificity This IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human IL5RA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL5RA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL5RA
Synonyms IL5R
Function This is the receptor for interleukin-5. The alpha chain binds to IL5.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed on eosinophils and basophils.
Research Areas
Citations (0)

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IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.


Matarin, M., et al. Stroke 40(11):3436-3442(2009)
Beekman, J.M., et al. Blood 114(18):3917-3927(2009)
Song, X.Y., et al. Diabetologia 52(8):1543-1553(2009)

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$ 295.00
$ 99.00
Cat# AP5235a
(40 western blots)
Availability: In Stock
Bulk Size
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