|Application ||WB, FC, E|
|Calculated MW||114225 Da|
|Antigen Region||146-174 aa|
|Other Names||Fanconi-associated nuclease 1, 3121-, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, FAN1, KIAA1018, MTMR15, MTMRF|
|Target/Specificity||This MTMRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the N-terminal region of human MTMRF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MTMR15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866).|
|Cellular Location||Nucleus Note=Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2 (PubMed:20603015, PubMed:20603016) Localizes to stalled replication forks via its UBZ-type zinc finger (PubMed:20935496).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
MTMRF encodes a member of the myotubularin-related class 1 cysteine-based protein tyrosine phosphatases. The encoded protein may be catalytically inactive.
Kimura, K., et al. Genome Res. 16(1):55-65(2006)
Suzuki, Y., et al. Genome Res. 14(9):1711-1718(2004)
Alonso, A., et al. Cell 117(6):699-711(2004)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.