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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   KAP1 / TIF1 beta Antibody   

KAP1 / TIF1 beta Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - KAP1 / TIF1 beta Antibody AP52671
    Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.
  • ICC - KAP1 / TIF1 beta Antibody AP52671
    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).
  • IP - KAP1 / TIF1 beta Antibody AP52671
    Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.
  • IHC - KAP1 / TIF1 beta Antibody AP52671
    IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.
  • SPECIFICATION
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, IP, IHC
Primary Accession Q13263
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 110 KDa
Additional Information
Gene ID 10155
Other Names E3 SUMO protein ligase TRIM28;E3 SUMO-protein ligase TRIM28;FLJ29029;KAP 1;KAP-1;KRAB associated protein 1;KRAB interacting protein 1;KRAB-associated protein 1;KRAB-interacting protein 1;KRIP 1;KRIP-1;KRIP1;Nuclear corepressor KAP 1;Nuclear corepressor KAP-1;RING finger protein 96;RNF96;TF1B;TIF1 beta;TIF1-beta;TIF1B;TIF1B_HUMAN; Transcription intermediary factor 1 beta;Transcription intermediary factor 1-beta; TRIM28;Tripartite motif containing 28;tripartite motif containing protein 28;Tripartite motif-containing protein 28.
Dilution WB~~1:1000
ICC~~1:100
IP~~1:500
IHC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TRIM28
Synonyms KAP1, RNF96, TIF1B
Function Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754).
Cellular Location Nucleus Note=Associated with centromeric heterochromatin during cell differentiation through CBX1.
Tissue Location Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Research Areas
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Background

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors.

References

Friedman J.R.,et al.Genes Dev. 10:2067-2078(1996).
Moosmann P.R.,et al.Nucleic Acids Res. 24:4859-4867(1996).
Emison E.S.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (MAY-2006) to UniProtKB.
Bienvenut W.V.,et al.Submitted (JAN-2010) to UniProtKB.

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$ 295.00
Cat# AP52671
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Quantity:
(40 western blots)
Availability: 5-7 days
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