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SPG16 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - SPG16 Antibody (Center) AP5274c
    SPG16 Antibody (Center) (Cat. #AP5274c) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPG16 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8N0X2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70818 Da
Antigen Region 276-304 aa
Additional Information
Gene ID 79582
Other Names Sperm-associated antigen 16 protein, Pf20 protein homolog, SPAG16, PF20
Target/Specificity This SPG16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human SPG16.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPG16 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPAG16
Synonyms PF20
Function Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, flagellum axoneme. Cytoplasm, cytoskeleton, cilium axoneme. Note=Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity)
Tissue Location Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver.
Research Areas
Citations (0)

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Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively.


Zhang, Z., et al. Biol. Reprod. 77(5):864-871(2007)
Zhang, Z., et al. Mol. Cell. Biol. 22(22):7993-8004(2002)
Pennarun, G., et al. Am. J. Respir. Cell Mol. Biol. 26(3):362-370(2002)

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$ 295.00
$ 99.00
Cat# AP5274c
(40 western blots)
Availability: In Stock
Bulk Size
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