|Application ||IHC-P, E|
|Calculated MW||70818 Da|
|Antigen Region||276-304 aa|
|Other Names||Sperm-associated antigen 16 protein, Pf20 protein homolog, SPAG16, PF20|
|Target/Specificity||This SPG16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human SPG16.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPG16 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, flagellum axoneme. Cytoplasm, cytoskeleton, cilium axoneme. Note=Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity)|
|Tissue Location||Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver.|
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Provided below are standard protocols that you may find useful for product applications.
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively.
Zhang, Z., et al. Biol. Reprod. 77(5):864-871(2007)
Zhang, Z., et al. Mol. Cell. Biol. 22(22):7993-8004(2002)
Pennarun, G., et al. Am. J. Respir. Cell Mol. Biol. 26(3):362-370(2002)
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