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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   p27 KIP1 Antibody   

p27 KIP1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - p27 KIP1 Antibody AP52854
    Western blot detection of p27 Kip1 in 3T3 cell lysates using p27 Kip1 mouse mAb (1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P46527
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 27 KDa
Additional Information
Gene ID 1027
Other Names AA408329;AI843786;Cdki1b;CDKN 1B;CDKN 4;CDKN1B;CDKN1B;CDKN4;CDKN4;CDN1B_HUMAN;Cyclin Dependent Kinase Inhibitor 1B;Cyclin Dependent Kinase Inhibitor 1B;Cyclin dependent kinase inhibitor p27;Cyclin dependent kinase inhibitor p27;Cyclin-dependent kinase inhibitor 1B (p27, Kip1);Cyclin-dependent kinase inhibitor 1B;Cyclin-dependent kinase inhibitor p27;Cyclin-dependent kinase inhibitor p27 Kip1;KIP 1;KIP1;MEN1B;MEN4;OTTHUMP00000195098;OTTHUMP00000195099;p27;p27 Kip1;P27-like cyclin-dependent kinase inhibitor;P27KIP1
Dilution WB~~1:1000
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CDKN1B
Synonyms KIP1
Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).
Tissue Location Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney
Research Areas
Citations (0)

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Background

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

References

Polyak K.,et al.Cell 78:59-66(1994).
Pietenpol J.A.,et al.Cancer Res. 55:1206-1210(1995).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Montagnoli A.,et al.Genes Dev. 13:1181-1189(1999).
Ishida N.,et al.J. Biol. Chem. 275:25146-25154(2000).

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$ 295.00
Cat# AP52854
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
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