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TSH2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TSH2 Antibody  (N-term) AP5288A
    Western blot analysis of TSH2 Antibody (N-term) (Cat. #AP5288a) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.
  • IHC-P - TSH2 Antibody  (N-term) AP5288A
    TSH2 Antibody (N-term) (Cat. #AP5288a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - TSH2 Antibody  (N-term) AP5288A
    TSH2 Antibody (N-term) (Cat. #AP5288a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - TSH2 Antibody  (N-term) AP5288A
    Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term)(Cat#AP5288a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NRE2
Other Accession A5GFT6, Q68FE9
Reactivity Human
Predicted Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 115005 Da
Antigen Region 232-261 aa
Additional Information
Gene ID 128553
Other Names Teashirt homolog 2, Ovarian cancer-related protein 10-2, OVC10-2, Zinc finger protein 218, TSHZ2, C20orf17, TSH2, ZNF218
Target/Specificity This TSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-261 amino acids from the N-terminal region of human TSH2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSH2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSHZ2
Synonyms C20orf17, TSH2, ZNF218
Function Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor (Potential).
Cellular Location Nucleus.
Tissue Location Expressed in brain; strongly reduced in post- mortem elderly subjects with Alzheimer disease
Citations (0)

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TSH2 is transcriptional regulator involved in developmental processes (Potential).


Jenkins, D., et al. Nephrol. Dial. Transplant. 25(1):54-60(2010)
Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Gocke, C.B., et al. J. Biol. Chem. 280(6):5004-5012(2005)

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Cat# AP5288A
(40 western blots)
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