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GNE Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GNE Antibody (C-term) AP5318B
    GNE Antibody (C-term) (Cat. #AP5318b) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNE antibody detected GNE protein (arrow).
  • FC - GNE Antibody (C-term) AP5318B
    GNE Antibody (C-term) (Cat. #AP5318b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y223
Other Accession O35826, Q91WG8, Q7TQ49, NP_005467.1
Reactivity Human, Mouse
Predicted Hamster, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79275 Da
Antigen Region 580-607 aa
Additional Information
Gene ID 10020
Other Names Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase (hydrolyzing), UDP-GlcNAc-2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, N-acetylmannosamine kinase, ManAc kinase, GNE, GLCNE
Target/Specificity This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 580-607 amino acids from the C-terminal region of human GNE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNE
Synonyms GLCNE
Function Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.
Cellular Location Cytoplasm.
Tissue Location Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.
Research Areas
Citations (0)

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GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.


Reinke, S.O., et al. Glycoconj. J. 26(4):415-422(2009)
Tong, Y., et al. PLoS ONE 4 (10), E7165 (2009)
Klootwijk, R.D., et al. FASEB J. 22(11):3846-3852(2008)

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Cat# AP5318B
(40 western blots)
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