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ACTL6B Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP5327a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP5327a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ACTL6B Antibody (N-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | O94805 |
| Other Accession | NP_057272.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 46877 Da |
ACTL6B Antibody (N-term) - Additional info | |
| Gene ID 51412 | |
| Other Names ACTL6B; ACTL6; BAF53B; Actin-like protein 6B; 53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B | |
| Target/Specificity This ACTL6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human ACTL6B. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACTL6B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACTL6B Antibody (N-term) - Protein Information | |
| Name ACTL6B | |
| Synonyms ACTL6, BAF53B | |
| Function Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity) | |
| Cellular Location Nucleus. | |
ACTL6B Antibody (N-term) - Related products
AP5327a: ACTL6B Antibody (N-term)
DC05017: Human ACTL6B cDNA Clone
ACTL6B Antibody (N-term) - Application data
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ACTL6B Antibody (N-term) (Cat. #AP5327a) western blot analysis in NCI-H460,HepG2 cell line lysates (35ug/lane).This demonstrates the ACTL6B antibody detected the ACTL6B protein (arrow).
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ACTL6B Antibody (N-term) (Cat. #AP5327a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTL6B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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ACTL6B Antibody (N-term) (Cat. #AP5327a) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ACTL6B Antibody (N-term) - Research Areas
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BACKGROUND
ACTL6B is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.
REFERENCES
Oma, Y., et al. Biochem. Biophys. Res. Commun. 301(2):521-528(2003) Kuroda, Y., et al. Biochem. Biophys. Res. Commun. 299(2):328-334(2002) Olave, I., et al. Genes Dev. 16(19):2509-2517(2002)
