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Anti-BiP/GRP78 Antibody

Mouse Anti Human Monoclonal Antibody

     
  • WB - Anti-BiP/GRP78 Antibody AP53389
    Western blot analysis of extracts from 3T3,A549,C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.
  • ICC - Anti-BiP/GRP78 Antibody AP53389
    Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20℃ and using BiP/GRP78 mouse mAb (dilution 1:200).
  • IP - Anti-BiP/GRP78 Antibody AP53389
    Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IP
Primary Accession P11021
Other Accession NM_005347
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Immunogen Purified recombinant human BiP/GRP78 protein expressed in E.coli.
Purification Affinity purified
Calculated MW 78 KDa
Additional Information
Gene ID 3309
Other Names 78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP;D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78;Endoplasmic reticulum lumenal Ca2+ binding protein grp78;FLJ26106;Glucose Regulated Protein 78kDa;GRP 78;GRP-78;GRP78;GRP78_HUMAN;Heat shock 70 kDa protein 5;Heat Shock 70kDa Protein 5;Hsce70;HSPA 5;HSPA5;Immunoglobulin Heavy Chain Binding Protein;Immunoglobulin heavy chain-binding protein;mBiP;MIF2;Sez7.
Dilution WB~~1:1000
Format PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HSPA5
Synonyms GRP78
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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Background

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate

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$ 295.00
Cat# AP53389
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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