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Anti-FAK Antibody

Mouse Anti Human Monoclonal Antibody

  • WB - Anti-FAK Antibody AP53408
    Western blot detection of FAK in COS7,HUVEC,A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.
  • ICC - Anti-FAK Antibody AP53408
    Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20℃ and using FAK mouse mAb (dilution 1:200).
  • IP - Anti-FAK Antibody AP53408
    Immunoprecipitation analysis of Hela cell lysates using FAK mouse mAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05397
Other Accession NM_153831.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Purified recombinant human FAK protein fragments expressed in E.coli.
Purification Affinity purified
Calculated MW 125 KDa
Additional Information
Gene ID 5747
Other Names FADK 1;FADK;FAK 1;FAK 1;FAK related non kinase polypeptide;FAK1;FAK1_HUMAN;Focal adhesion kinase 1;Focal adhesion kinase 1;Focal adhesion Kinase;Focal adhesion kinase isoform FAK Del33;Focal adhesion kinase related nonkinase;FRNK;p125FAK;pp125FAK;pp125FAK;PPP1R71;Protein phosphatase 1 regulatory subunit 71;Protein tyrosine kinase 2;Protein Tyrosine Kinase Cytoplasmic;Protein Tyrosine Kinase Cytoplasmic;Protein-tyrosine kinase 2;PTK 2;PTK 2;PTK2;PTK2 protein tyrosine kinase 2;PTK2 protein tyrosine kinase 2.
Dilution WB~~1:1000
Format PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PTK2
Synonyms FAK, FAK1
Function Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=Constituent of focal adhesions Detected at microtubules
Tissue Location Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level) Ubiquitous.
Research Areas
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Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell

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$ 295.00
Cat# AP53408
(40 western blots)
Availability: 5-7 days
Bulk Size
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