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>   home   >   Products   >   Primary Antibodies   >   EIF3G Antibody (Center)   

EIF3G Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF3G Antibody (Center) AP5342c
    EIF3G Antibody (Center) (Cat. #AP5342c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).
  • IHC-P - EIF3G Antibody (Center) AP5342c
    EIF3G Antibody (Center) (Cat. #AP5342c) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - EIF3G Antibody (Center) AP5342c
    EIF3G Antibody (Center) (Cat. #AP5342c) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O75821
Other Accession Q5RK09, Q9Z1D1, Q6DRC4, Q3ZC12, Q641B2, Q6DJI8, NP_003746.2
Reactivity Human
Predicted Xenopus, Bovine, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35611 Da
Antigen Region 209-238 aa
Additional Information
Gene ID 8666
Other Names Eukaryotic translation initiation factor 3 subunit G {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3g {ECO:0000255|HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}, eIF-3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 subunit 4 {ECO:0000255|HAMAP-Rule:MF_03006}, eIF-3-delta {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3 p42 {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3 p44 {ECO:0000255|HAMAP-Rule:MF_03006}, EIF3G {ECO:0000255|HAMAP-Rule:MF_03006}
Target/Specificity This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the Central region of human EIF3G.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF3G Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3G {ECO:0000255|HAMAP-Rule:MF_03006}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03006}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17094969}. Cytoplasm, perinuclear region {ECO:0000255|HAMAP-Rule:MF_03006, ECO:0000269|PubMed:17094969} Note=Colocalizes with AIFM1 in the nucleus and perinuclear region
Citations (0)

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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.

References

Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)

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Cat# AP5342c
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