|Application ||WB, IHC-P, E|
|Other Accession||Q5FWT9, Q0VCF3, NP_001095866.1, NP_079349.2|
|Calculated MW||23721 Da|
|Antigen Region||154-182 aa|
|Other Names||Suppressor of IKBKE 1, Suppressor of IKK-epsilon, SIKE1, SIKE|
|Target/Specificity||This SIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human SIKE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SIKE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.|
|Tissue Location||Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).
Goudreault, M., et al. Mol. Cell Proteomics 8(1):157-171(2009)
Huang, J., et al. EMBO J. 24(23):4018-4028(2005)
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