|Application ||WB, IHC-P, FC, E|
|Other Accession||NP_958440.1, NP_005219.2|
|Calculated MW||134277 Da|
|Antigen Region||1004-1033 aa|
|Other Names||Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1|
|Target/Specificity||This EGFR-S1026 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1004-1033 amino acids from the C-terminal region of human EGFR-S1026.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EGFR-S1026 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERBB, ERBB1, HER1|
|Function||Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)|
|Tissue Location||Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Perez, C.A., et al. J. Urol. 183(5):2062-2069(2010)
Koumakpayi, I.H., et al. Br. J. Cancer 102(7):1163-1173(2010)
Cortot, A.B., et al. Cancer (2010) In press :
Lee, Y.J., et al. J. Cancer Res. Clin. Oncol. (2010) In press :
Kawahara, A., et al. Hum. Pathol. (2010) In press :
Wu, S.L., et al. Mol. Cell Proteomics 5(9):1610-1627(2006)
Wu, S.L., et al. J. Proteome Res. 4(4):1155-1170(2005)
Abe, Y., et al. J. Biol. Chem. 273(18):11150-11157(1998)
Li, W., et al. Mol. Cell. Biol. 12(12):5824-5833(1992)
Krieg, J., et al. J. Biol. Chem. 267(27):19258-19265(1992)
Lowenstein, E.J., et al. Cell 70(3):431-442(1992)
Chi, D.D., et al. Hum. Mol. Genet. 1 (2), 135 (1992) :
Countaway, J.L., et al. J. Biol. Chem. 267(2):1129-1140(1992)
If you have any additional inquiries please email technical services at email@example.com.