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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NR3C1 Antibody (Center)   

NR3C1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NR3C1 Antibody (Center) AP5468c
    NR3C1 Antibody (Center) (Cat.#AP5468c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).
  • IHC-P - NR3C1 Antibody (Center) AP5468c
    NR3C1 Antibody (Center) (Cat. #AP5468c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR3C1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - NR3C1 Antibody (Center) AP5468c
    Fluorescent confocal image of Hela cell stained with NR3C1 Antibody (Center)(Cat#AP5468c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P04150
Other Accession P59667, NP_000167.1
Reactivity Human, Mouse
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85659 Da
Antigen Region 236-262 aa
Additional Information
Gene ID 2908
Other Names Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Target/Specificity This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-262 amino acids from the Central region of human NR3C1.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNR3C1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR3C1
Synonyms GRL
Function Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).
Cellular Location Isoform Alpha: Cytoplasm. Nucleus Mitochondrion. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=After ligand activation, translocates from the cytoplasm to the nucleus Isoform Alpha-B: Nucleus. Cytoplasm. Note=After ligand activation, translocates from the cytoplasm to the nucleus
Tissue Location Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart (PubMed:10902803). Isoform Beta: Widely expressed including brain, bone marrow, thymus, spleen, liver, kidney, pancreas, lung, fat, skeletal muscle, heart, placenta and blood leukocytes (PubMed:7769088, PubMed:8621628). Isoform Alpha-2: Expressed at low level.
Research Areas
Citations (0)

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Background

NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.

References

Bosker, F.J., et al. Mol. Psychiatry (2010)
Inkster, B., et al. Neuroimage (2010)
Geelhoed, M.J., et al. BMC Med. Genet. 11, 39 (2010)

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$ 295.00
$ 99.00
Cat# AP5468c
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(40 western blots)
Availability: In Stock
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