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PMCH Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PMCH Antibody (Center) AP5528c
    PMCH Antibody (Center) (Cat. #AP5528c) western blot analysis in K562 cell line lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).
  • WB - PMCH Antibody (Center) AP5528c
    PMCH Antibody (Center) (Cat. #AP5528c) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the PMCH antibody detected the PMCH protein (arrow).
  • IHC-P - PMCH Antibody (Center) AP5528c
    PMCH Antibody (Center) (Cat. #AP5528c) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PMCH Antibody (Center) AP5528c
    PMCH Antibody (Center) (Cat. #AP5528c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - PMCH Antibody (Center) AP5528c
    Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center) (Cat. #AP5528c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20382
Other Accession NP_002665.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18679 Da
Antigen Region 94-122 aa
Additional Information
Gene ID 5367
Other Names Pro-MCH, Neuropeptide-glycine-glutamic acid, NGE, Neuropeptide G-E, Neuropeptide-glutamic acid-isoleucine, NEI, Neuropeptide E-I, Melanin-concentrating hormone, MCH, PMCH, MCH
Target/Specificity This PMCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the Central region of human PMCH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPMCH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MCH
Function MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
Cellular Location Secreted.
Tissue Location Predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum Also found in thymus, brown adipose tissue, duodenum and testis (spermatogonia, early spermatocytes and Sertoli cells). No expression in peripheral blood. In brain exclusively mature MCH and NEI peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly.
Research Areas
Citations (0)

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The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus (summarized by Nahon et al., 1992 [PubMed 1572663]). In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).


Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
Aziz, A., et al. Brain Pathol. 18(4):474-483(2008)
Kokkotou, E., et al. Proc. Natl. Acad. Sci. U.S.A. 105(30):10613-10618(2008)

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Cat# AP5528c
(40 western blots)
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