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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   MARCH5 Antibody (N-term)   

MARCH5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MARCH5 Antibody (N-term) AP5677a
    Western blot analysis of MARCH5 Antibody (N-term) (Cat. #AP5677a) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.
  • IHC-P - MARCH5 Antibody (N-term) AP5677a
    MARCH5 Antibody (N-term) (Cat. #AP5677a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - MARCH5 Antibody (N-term) AP5677a
    MARCH5 Antibody (N-term) (Cat. #AP5677a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NX47
Other Accession Q6GM44, Q3KNM2, Q5ZJ41, Q3ZC24, NP_060294
Reactivity Human, Mouse
Predicted Bovine, Chicken, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31232 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 54708
Other Names E3 ubiquitin-protein ligase MARCH5, 632-, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, MARCH-V, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, MARCH5, RNF153
Target/Specificity This MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARCH5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RNF153
Function Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301)
Tissue Location Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle
Research Areas
Citations (0)

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MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).


Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009) Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007) Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006)

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Cat# AP5677a
(40 western blots)
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