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MARCH5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MARCH5 Antibody (N-term) AP5677a
    All lanes : Anti-MARCH5 Antibody (N-term) at 1:1000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: T47D whole cell lysate Lane 3: Human heart lysate Lane 4: Mouse kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NX47
Other Accession Q6GM44, Q3KNM2, Q5ZJ41, Q3ZC24, NP_060294
Reactivity Human, Mouse
Predicted Bovine, Chicken, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31232 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 54708
Other Names E3 ubiquitin-protein ligase MARCH5, 632-, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, MARCH-V, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, MARCH5, RNF153
Target/Specificity This MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARCH5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MARCH5
Synonyms RNF153
Function Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).
Tissue Location Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle
Research Areas
Citations (0)

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Background

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).

References

Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009) Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007) Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006)

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$ 295.00
$ 99.00
Cat# AP5677a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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