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BHLH3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - BHLH3 Antibody (N-term) AP5748a
    Anti-BHLH3 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - BHLH3 Antibody (N-term) AP5748a
    BHLH3 Antibody (N-term) (Cat. #AP5748a) western blot analysis in RD cell line lysates (35ug/lane).This demonstrates the BHE41 antibody detected the BHE41 protein (arrow).
  • FC - BHLH3 Antibody (N-term) AP5748a
    BHLH3 Antibody (N-term) (Cat. #AP5748a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - BHLH3 Antibody (N-term) AP5748a
    Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term)(Cat#AP5748a). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9C0J9
Other Accession NP_110389
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50498 Da
Antigen Region 17-46 aa
Additional Information
Gene ID 79365
Other Names Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, bHLHb3, Differentially expressed in chondrocytes protein 2, hDEC2, Enhancer-of-split and hairy-related protein 1, SHARP-1, BHLHE41, BHLHB3, DEC2, SHARP1
Target/Specificity This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBHLH3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BHLHE41
Synonyms BHLHB3, DEC2, SHARP1
Function Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Tissue Location Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney
Research Areas
Citations (0)

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BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.


Honma, S., et al. Nature 419(6909):841-844(2002) Garriga-Canut, M., et al. J. Biol. Chem. 276(18):14821-14828(2001) Fujimoto, K., et al. Biochem. Biophys. Res. Commun. 280(1):164-171(2001) Grottke, C., et al. Int. J. Cancer 88(4):535-546(2000)

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$ 295.00
$ 99.00
Cat# AP5748a
(40 western blots)
Availability: In Stock
Bulk Size
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