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SOX10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - SOX10 Antibody (Center) AP5854C
    AP5854c staining SOX10 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - SOX10 Antibody (Center) AP5854C
    AP5854c staining SOX10 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - SOX10 Antibody (Center) AP5854C
    All lanes : Anti-SOX10 Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysates Lane 2: C6 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SOX10 Antibody (Center) AP5854C
    SOX10 Antibody (Center) (Cat. #AP5854c) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the SOX10 antibody detected SOX10 protein (arrow).
  • IHC-P - SOX10 Antibody (Center) AP5854C
    SOX10 Antibody (Center) (Cat. #AP5854c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOX10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P56693
Other Accession O55170, A5A763, Q04888, NP_008872.1
Reactivity Human, Mouse, Rat
Predicted Rat, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49911 Da
Antigen Region 231-259 aa
Additional Information
Gene ID 6663
Other Names Transcription factor SOX-10, SOX10
Target/Specificity This SOX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-259 amino acids from the Central region of human SOX10.
Dilution IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOX10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX10
Function Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia (By similarity).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in fetal brain and in adult brain, heart, small intestine and colon
Research Areas
Citations ( 0 )
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$ 295.00
$ 99.00
Cat# AP5854C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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