|Application ||WB, IHC-P, E|
|Other Accession||P61206, P61205, P61204, Q5E9I6, P84082, Q8BSL7, P84081, P51643, P84079, P84078, Q4R5P2, P84080, NP_001019398.1, NP_001649.1, NP_001019399.1, NP_001019397.1|
|Predicted||Bovine, Monkey, Mouse, Rat, Xenopus|
|Calculated MW||20697 Da|
|Antigen Region||80-106 aa|
|Other Names||ADP-ribosylation factor 1, ARF1|
|Target/Specificity||This ARF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-106 amino acids from the Central region of human ARF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).|
|Cellular Location||Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density|
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