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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   XIAP (BIRC4) Antibody (N-term)   

XIAP (BIRC4) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - XIAP (BIRC4) Antibody (N-term) AP6125a
    Western blot analysis of anti-BIRC4 Antibody (N-term) (Cat.#AP6125a) in Ramos cell line lysates (35ug/lane). BIRC4(arrow) was detected using the purified Pab.
  • WB - XIAP (BIRC4) Antibody (N-term) AP6125a
    The anti-BIRC4 (N-term) Pab (Cat. #AP6125a) is used in Western blot to detect BIRC4 in mouse liver tissue lysate.
  • IHC-P - XIAP (BIRC4) Antibody (N-term) AP6125a
    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with BIRC4 antibody (N-term) (Cat.#AP6125a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P98170
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56685 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 331
Other Names E3 ubiquitin-protein ligase XIAP, 632-, Baculoviral IAP repeat-containing protein 4, IAP-like protein, ILP, hILP, Inhibitor of apoptosis protein 3, IAP-3, hIAP-3, hIAP3, X-linked inhibitor of apoptosis protein, X-linked IAP, XIAP, API3, BIRC4, IAP3
Target/Specificity This XIAP (BIRC4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human XIAP (BIRC4).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsXIAP (BIRC4) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms API3, BIRC4, IAP3
Function Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta- catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.
Cellular Location Cytoplasm. Nucleus. Note=TLE3 promotes its nuclear localization
Tissue Location Ubiquitous, except peripheral blood leukocytes
Research Areas
Citations (0)

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BIRC4 is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Similar to API1, BIRC4 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.


Srinivasula, S.M., et al., J. Biol. Chem. 278(34):31469-31472 (2003). Miranda, M.B., et al., Leukemia 17(2):390-400 (2003). Ahn, S.K., et al., Int. J. Mol. Med. 12(2):231-236 (2003). Yu, L.Y., et al., Mol. Cell. Neurosci. 22(3):308-318 (2003). Nomura, T., et al., Urol. Res. 31(1):37-44 (2003).

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$ 295.00
$ 99.00
Cat# AP6125a
(40 western blots)
Availability: In Stock
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