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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GREMLIN Antibody (C-term)   

GREMLIN Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GREMLIN Antibody (C-term) AP6133a
    Western blot analysis of anti-Gremlin Pab (Cat. #AP6133a) in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab.
  • WB - GREMLIN Antibody (C-term) AP6133a
    Western blot analysis of anti-Gremlin Pab (Cat. #AP6133a) in mouse liver cell lysate. Gremlin (arrow) was detected by using AP6133a. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - GREMLIN Antibody (C-term) AP6133a
    Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.
  • IHC-P - GREMLIN Antibody (C-term) AP6133a
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody (C-term) (Cat.#AP6133a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - GREMLIN Antibody (C-term) AP6133a
    ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O70326
Other Accession O35793, O60565
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20710 Da
Antigen Region 147-175 aa
Additional Information
Gene ID 23892
Other Names Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm
Target/Specificity This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of mouse GREMLIN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGREMLIN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Grem1
Synonyms Cktsf1b1, Drm
Function Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.
Cellular Location Secreted.
Tissue Location Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non- transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4
Research Areas
Citations ( 0 )


GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.


Chen, B., et al., J. Immunol. 173(10):5914-5917 (2004). Chen, B., et al., Biochem. Biophys. Res. Commun. 295(5):1135-1141 (2002). McMahon, R., et al., J. Biol. Chem. 275(14):9901-9904 (2000). Murphy, M., et al., J. Biol. Chem. 274(9):5830-5834 (1999). Hsu, D.R., et al., Mol. Cell 1(5):673-683 (1998).

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$ 295.00
$ 99.00
Cat# AP6133a
(40 western blots)
Availability: In Stock
Bulk Size
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