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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   MAGEA4 Antibody (N-term)   

MAGEA4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAGEA4 Antibody (N-term) AP6166a
    The anti-MAGE-A4 Pab (Cat. #AP6166a) is used in Western blot to detect MAGE-A4 in A375 cell lysate.
  • IHC-P - MAGEA4 Antibody (N-term) AP6166a
    Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody Cat# AP6166a (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska?Institutet and University Hospital, Sweden.
  • IHC-P - MAGEA4 Antibody (N-term) AP6166a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) (Cat.#AP6166a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P43358
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 9-38 aa
Additional Information
Other Names Melanoma-associated antigen 4, Cancer/testis antigen 14, CT14, MAGE-4 antigen, MAGE-41 antigen, MAGE-X2 antigen, MAGEA4, MAGE4
Target/Specificity This MAGEA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human MAGEA4.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAGEA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAGEA4
Synonyms MAGE4
Function Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue Location Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta EMBL; U10687; AAA68871.1; -; Genomic_DNA EMBL; U10688; AAA68872.1; -; Genomic_DNA EMBL; U10340; AAA19007.1; -; mRNA EMBL; D32075; BAA06841.1; -; mRNA EMBL; D32077; BAA06843.1; -; mRNA EMBL; AF274855; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC017723; AAH17723.1; -; mRNA CCDS; CCDS14702.1; - PIR; I38661; I38661 RefSeq; NP_001011548.1; NM_001011548.1 RefSeq; NP_001011549.1; NM_001011549.1 RefSeq; NP_001011550.1; NM_001011550.1 RefSeq; NP_002353.3; NM_002362.4 RefSeq; XP_005274735.1; XM_005274678.3 RefSeq; XP_005274736.1; XM_005274679.3 UniGene; Hs.37107; - PDB; 1I4F; X-ray; 1.40 A; C=230-239 PDB; 2WA0; X-ray; 2.30 A; A=101-317 PDBsum; 1I4F; - PDBsum; 2WA0; - ProteinModelPortal; P43358; - SMR; P43358; - BioGrid; 110277; 33 IntAct; P43358; 21 MINT; P43358; - STRING; 9606.ENSP00000276344; - iPTMnet; P43358; - PhosphoSitePlus; P43358; - BioMuta; MAGEA4; - DMDM; 322510046; - EPD; P43358; - MaxQB; P43358; - PaxDb; P43358; - PeptideAtlas; P43358; - PRIDE; P43358; - ProteomicsDB; 55620; - DNASU; 4103; - Ensembl; ENST00000276344; ENSP00000276344; ENSG00000147381 Ensembl; ENST00000360243; ENSP00000353379; ENSG00000147381 Ensembl; ENST00000370335; ENSP00000359360; ENSG00000147381 Ensembl; ENST00000370340; ENSP00000359365; ENSG00000147381 Ensembl; ENST00000393920; ENSP00000377497; ENSG00000147381 Ensembl; ENST00000393921; ENSP00000377498; ENSG00000147381 GeneID; 4103; - KEGG; hsa:4103; - UCSC; uc004ffa.3; human CTD; 4103; - DisGeNET; 4103; - EuPathDB; HostDB:ENSG00000147381.11; - GeneCards; MAGEA4; - HGNC; HGNC:6802; MAGEA4 HPA; CAB040535; - HPA; HPA021942; - MIM; 300175; gene neXtProt; NX_P43358; - OpenTargets; ENSG00000147381; - PharmGKB; PA30548; - eggNOG; KOG4562; Eukaryota eggNOG; ENOG4111S70; LUCA GeneTree; ENSGT00760000118824; - HOGENOM; HOG000231161; - HOVERGEN; HBG006315; - InParanoid; P43358; - OMA; WEELGVM; - OrthoDB; EOG091G0TJ0; - PhylomeDB; P43358; - TreeFam; TF328505; - ChiTaRS; MAGEA4; human EvolutionaryTrace; P43358; - GeneWiki; MAGEA4; - GenomeRNAi; 4103; - PRO; PR:P43358; - Proteomes; UP000005640; Chromosome X Bgee; ENSG00000147381; - CleanEx; HS_MAGEA4; - ExpressionAtlas; P43358; baseline and differential Genevisible; P43358; HS InterPro; IPR037445; MAGE InterPro; IPR021072; MAGE_N InterPro; IPR030098; MAGEA4/MAGEA5 InterPro; IPR002190; MHD_dom PANTHER; PTHR11736; PTHR11736; 1 PANTHER; PTHR11736:SF48; PTHR11736:SF48; 1 Pfam; PF01454; MAGE; 1 Pfam; PF12440; MAGE_N; 1 SMART; SM01373; MAGE; 1 SMART; SM01392; MAGE_N; 1 PROSITE; PS50838; MAGE; 1 1: Evidence at protein level; 3D-structure; Complete proteome; Polymorphism; Reference proteome; Tumor antigen CHAIN 1 317 Melanoma-associated antigen 4 /FTId=PRO_0000156704 DOMAIN 110 309 MAGE. {ECO:0000255|PROSITE- ProRule:PRU00127} COMPBIAS 41 44 Poly-Ser VARIANT 137 137 L -> Q. /FTId=VAR_076262 VARIANT 153 153 G -> D (in a breast cancer sample; somatic mutation) /FTId=VAR_036582 VARIANT 173 173 A -> T (in dbSNP:rs1047251) /FTId=VAR_004284 VARIANT 230 230 G -> E (in dbSNP:rs773875619) /FTId=VAR_078319 CONFLICT 3 3 S -> L (in Ref. 3; BAA06841) CONFLICT 48 48 P -> L (in Ref. 3; BAA06841) CONFLICT 53 53 E -> K (in Ref. 3; BAA06841) CONFLICT 61 61 G -> D (in Ref. 3; BAA06841) CONFLICT 95 96 GP -> EA (in Ref. 3; BAA06841) CONFLICT 307 307 E -> Q (in Ref. 2; AAA19007) HELIX 102 126 {ECO:0000244|PDB:2WA0} HELIX 133 139 {ECO:0000244|PDB:2WA0} HELIX 145 147 {ECO:0000244|PDB:2WA0} HELIX 148 163 {ECO:0000244|PDB:2WA0} STRAND 165 169 {ECO:0000244|PDB:2WA0} STRAND 176 181 {ECO:0000244|PDB:2WA0} HELIX 182 184 {ECO:0000244|PDB:2WA0} HELIX 200 213 {ECO:0000244|PDB:2WA0} HELIX 219 228 {ECO:0000244|PDB:2WA0} TURN 238 240 {ECO:0000244|PDB:2WA0} HELIX 243 248 {ECO:0000244|PDB:2WA0} HELIX 250 253 {ECO:0000244|PDB:2WA0} STRAND 256 260 {ECO:0000244|PDB:2WA0} STRAND 271 274 {ECO:0000244|PDB:2WA0} HELIX 276 281 {ECO:0000244|PDB:2WA0} HELIX 284 295 {ECO:0000244|PDB:2WA0} HELIX 305 309 {ECO:0000244|PDB:2WA0} SEQUENCE 317 AA; 34899 MW; C6AA2EE306963E7D CRC64; MSSEQKSQHC KPEEGVEAQE EALGLVGAQA PTTEEQEAAV SSSSPLVPGT LEEVPAAESA GPPQSPQGAS ALPTTISFTC WRQPNEGSSS QEEEGPSTSP DAESLFREAL SNKVDELAHF LLRKYRAKEL VTKAEMLERV IKNYKRCFPV IFGKASESLK MIFGIDVKEV DPASNTYTLV TCLGLSYDGL LGNNQIFPKT GLLIIVLGTI AMEGDSASEE EIWEELGVMG VYDGREHTVY GEPRKLLTQD WVQENYLEYR QVPGSNPARY EFLWGPRALA ETSYVKVLEH VVRVNARVRI AYPSLREAAL LEEEEGV
Research Areas
Citations ( 0 )

Background

MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

References

Nagao, T., et al., J. Biol. Chem. 278(12):10668-10674 (2003).
Resnick, M.B., et al., Int. J. Cancer 101(2):190-195 (2002).
Imai, Y., et al., Gene 160(2):287-290 (1995).
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

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$ 295.00
$ 99.00
Cat# AP6166a
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