|Application ||WB, IHC-P, E|
|Calculated MW||53684 Da|
|Antigen Region||44-73 aa|
|Other Names||Forkhead box protein O4, Fork head domain transcription factor AFX1, FOXO4, AFX, AFX1, MLLT7|
|Target/Specificity||This MLLT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human MLLT7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MLLT7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AFX, AFX1, MLLT7|
|Function||Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The MLLT7 (Mixed-lineage leukemia translocated to 7) protein is a forkhead transcription factor that activates apoptosis by inducing the BCL-6 transcriptional repressor. MLL-MLLT7 fusion protein transformw myeloid progenitors and impairs forkhead protein function. MLLT7 is a target of the phosphatidylinositol 3-kinase/PKB insulin signaling pathway and the AMP-activated protein kinase-dependent pathway.
Crossley, L.J., J. Leukoc. Biol. 74(4):583-592 (2003).
Tang, T.T., et al., J. Biol. Chem. 278(32):30125-30135 (2003).
So, C.W., et al., Mol. Cell. Biol. 22(18):6542-6552 (2002).
Tang, T.T., et al., J. Biol. Chem. 277(16):14255-14265 (2002).
Kops, G.J., et al., Mol. Cell. Biol. 22(7):2025-2036 (2002).
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