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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MMP9 Antibody (C-term)   

MMP9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MMP9 Antibody (C-term) AP6214a
    All lanes : Anti-MMP9 Antibody (L659) at 1:2000 dilution Lane 1: NCI-H1299 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human Spleen tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 8
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P14780
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78458 Da
Antigen Region 644-673 aa
Additional Information
Gene ID 4318
Other Names Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B
Target/Specificity This MMP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP9
Synonyms CLG4B
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Produced by normal alveolar macrophages and granulocytes
Research Areas
Citations ( 0 )

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

References

Tutton, M.G., et al., Int. J. Cancer 107(4):541-550 (2003). Behrens, P., et al., Int. J. Cancer 107(2):183-188 (2003). Van den Steen, P.E., et al., Biochem. Biophys. Res. Commun. 310(3):889-896 (2003). Zhao, Z., et al., J. Infect. Dis. 188(8):1098-1104 (2003). Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003).

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$ 295.00
$ 99.00
Cat# AP6214a
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(40 western blots)
Availability: In Stock
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