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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   SIRT5 Antibody (C-term)   

SIRT5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - SIRT5 Antibody (C-term) AP6244a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - SIRT5 Antibody (C-term) AP6244a
    Western blot analysis of anti-SIRT5 Pab (Cat. #AP6244a) in Jurkat cell line lysate (35ug/lane). SIRT5(arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NXA8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33881 Da
Antigen Region 263-292 aa
Additional Information
Gene ID 23408
Other Names NAD-dependent protein deacylase sirtuin-5, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03160}, 351- {ECO:0000255|HAMAP-Rule:MF_03160}, Regulatory protein SIR2 homolog 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2-like protein 5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}, SIR2L5
Target/Specificity This SIRT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human SIRT5.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT5 {ECO:0000255|HAMAP-Rule:MF_03160}
Synonyms SIR2L5
Function NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.
Cellular Location Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially: a fraction is present in the cytosol and very small amounts are also detected in the nucleus Isoform 2: Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03160, ECO:0000269|PubMed:21143562}
Tissue Location Widely expressed.
Research Areas
Citations (0)

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SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.


Frye, R.A., Biochem. Biophys. Res. Commun. 273(2):793-798 (2000).
Frye, R.A., Biochem. Biophys. Res. Commun. 260(1):273-279 (1999).

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$ 295.00
$ 99.00
Cat# AP6244a
(40 western blots)
Availability: In Stock
Bulk Size
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