|Application ||WB, IF, IHC-P, E|
|Calculated MW||106159 Da|
|Antigen Region||586-615 aa|
|Other Names||Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1|
|Target/Specificity||This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from human RB1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RB1 Antibody (S608) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.|
|Tissue Location||Expressed in the retina.|
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Provided below are standard protocols that you may find useful for product applications.
The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer. One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.
Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004).
Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004).
Borah, S., et al., J. Virol. 78(19):10336-10347 (2004).
Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).
Lohmann, D.R., et al., J. Biol. Chem. 129(1):23-28 (2004).
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