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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CCNE1 Antibody (C-term)   

CCNE1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCNE1 Antibody (C-term) AP6270b
    Anti-Cyclin E1 Antibody at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CCNE1 Antibody (C-term) AP6270b
    Formalin-fixed and paraffin-embedded human testis tissue with Cyclin E1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CCNE1 Antibody (C-term) AP6270b
    Flow cytometric analysis of NCI-H292 cells using Cyclin E1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
Anti-Cyclin E1 Antibody at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P24864
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47077 Da
Antigen Region 373-402 aa
Additional Information
Gene ID 898
Other Names G1/S-specific cyclin-E1, CCNE1, CCNE
Target/Specificity This CCNE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the C-terminal region of human CCNE1.
Dilution FC~~1:10~50
IHC-P~~1:50~100
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNE1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNE1
Synonyms CCNE
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Cellular Location Nucleus.
Tissue Location Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Research Areas
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$ 385.00
Cat# AP6270b
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