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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD14 Antibody (N-term)   

CD14 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CD14 Antibody (N-term) AP6294A
    Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat.#AP6294a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
  • WB - CD14 Antibody (N-term) AP6294A
    CD14 Antibody (N-term) (Cat.#AP6294a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
  • IHC-P - CD14 Antibody (N-term) AP6294A
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat.#AP6294a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - CD14 Antibody (N-term) AP6294A
    Confocal immunofluorescent analysis of CD14 Antibody (N-term)(Cat#AP6294a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - CD14 Antibody (N-term) AP6294A
    CD14 Antibody (N-term) (Cat. #AP6294a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08571
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40076 Da
Antigen Region 54-83 aa
Additional Information
Gene ID 929
Other Names Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Target/Specificity This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD14 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD14
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages
Research Areas
Citations ( 0 )


CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.


Donati,M., J. Periodontol. 79 (3), 517-524 (2008)
Yuan,F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008)
Setoguchi,M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989)
Goyert,S.M., Science 239 (4839), 497-500 (1988)

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$ 295.00
$ 99.00
Cat# AP6294A
(40 western blots)
Availability: In Stock
Bulk Size
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