|Application ||WB, IHC-P, E|
|Calculated MW||58143 Da|
|Antigen Region||108-138 aa|
|Other Names||Signal peptide peptidase-like 2A, SPP-like 2A, SPPL2a, 3423-, Intramembrane protease 3, IMP-3, Presenilin-like protein 2, SPPL2A, IMP3, PSL2|
|Target/Specificity||This SPPL2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the N-terminal region of human SPPL2a.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPPL2a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity.|
|Cellular Location||Late endosome membrane; Multi- pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cell membrane. Note=Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane|
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Provided below are standard protocols that you may find useful for product applications.
By searching sequence databases for homologs of Dictyostelium discoideum Impas, which shares homology with presenilin, followed by PCR of lymphocyte and hippocampus cDNA libraries, Grigorenko et al. (2002) cloned SPPL2a. This protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus.
Grigorenko, A. P., et al. Biochemistry 67: 826-834 (2002).
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