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SPPL3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SPPL3 Antibody (N-term) AP6313a
    Western blot analysis of SPPL3(arrow) using rabbit polyclonal SPPL3 Antibody (N-term) (Cat.#AP6313a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPPL3 gene (Lane 2) (Origene Technologies).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8TCT6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42563 Da
Antigen Region 50-81 aa
Additional Information
Gene ID 121665
Other Names Signal peptide peptidase-like 3, SPP-like 3, 3423-, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3, IMP2, PSL4
Target/Specificity This SPPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-81 amino acids from the N-terminal region of human SPPL3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPPL3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPPL3 {ECO:0000303|PubMed:12077416, ECO:0000312|HGNC:HGNC:30424}
Function Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571). Acts like a sheddase by mediating the proteolytic release and secretion of active site- containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin- NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus Membrane; Multi-pass membrane protein; Lumenal side
Tissue Location Widely expressed (PubMed:15385547). Expressed in the brain (PubMed:11978763).
Research Areas
Citations (0)

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Background

Signal peptide peptidase (SPP) is an aspartyl protease that mediates clearance of signal peptides by proteolysis within the endoplasmic reticulum (ER). Like presenilins, SPP contains a critical GXGD motif in its C-terminal catalytic center. SPPL3 is one of several presenilin homologues/SPP-like proteins (PSHs/SPPL) that have been identified.

References

Grigorenko, A.P., et al., Biochemistry Mosc. 67(7):826-835 (2002).
Weihofen, A., et al., Science 296(5576):2215-2218 (2002).

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$ 295.00
$ 99.00
Cat# AP6313a
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Quantity:
(40 western blots)
Availability: In Stock
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