- CITATIONS: 2
|Application ||WB, E|
|Calculated MW||62769 Da|
|Antigen Region||237-266 aa|
|Other Names||Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, PTEN-induced putative kinase protein 1, PINK1|
|Target/Specificity||This PINK1 (PARK6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human PINK1 (PARK6).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PINK1 (PARK6) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291).|
|Cellular Location||Mitochondrion outer membrane; Single-pass membrane protein Cytoplasm, cytosol. Note=Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol|
|Tissue Location||Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development|
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Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson's disease 6 (PARK6). Six novel pathogenic PINK1 mutations suggest that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance. Strong evidence indicates that, although important in mendelian forms of Parkinson's disease (PD), PINK1 does not influence the cause of sporadic nonmendelian forms of PD.
Rogaeva,E.,et al. Arch. Neurol. 61 (12), 1898-1904 (2004) Hatano,Y., et al. Ann. Neurol. 56 (3), 424-427 (2004) Healy,D.G., et al. Ann. Neurol. 56 (3), 329-335 (2004) Valente,E.M., et al. Science 304 (5674), 1158-1160 (2004) Nakajima,A., et al. Cancer Lett. 201 (2), 195-201 (2003) Unoki,M. and Nakamura,Y. Oncogene 20 (33), 4457-4465 (2001)
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