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AGXT Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - AGXT Antibody (Center) AP6500c
    AP6500c staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - AGXT Antibody (Center) AP6500c
    Anti-AGXT Antibody (Center)at 1:8000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AGXT Antibody (Center) AP6500c
    Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AGXT Antibody (Center) AP6500c
    Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AGXT Antibody (Center) AP6500c
    Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AGXT Antibody (Center) AP6500c
    Western blot analysis of AGXT Antibody (Center) (Cat. #AP6500c) in HepG2 cell line lysates (35ug/lane). AGXT (arrow) was detected using the purified Pab.(8ug/ml)
  • IHC-P - AGXT Antibody (Center) AP6500c
    AGXT Antibody (Center) (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AGXT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - AGXT Antibody (Center) AP6500c
    AGXT Antibody (Center) (Cat. #AP6500c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P21549
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43010 Da
Antigen Region 96-125 aa
Additional Information
Gene ID 189
Other Names Serine--pyruvate aminotransferase, SPT, Alanine--glyoxylate aminotransferase, AGT, AGXT, AGT1, SPAT
Target/Specificity This AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.
Dilution IHC-P~~1:50~100
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGXT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGXT
Synonyms AGT1, SPAT
Cellular Location Peroxisome. Mitochondrion Note=Predominantly localized in the peroxisomes. Mitochondrial mistargeting occurs in variant proteins Arg-41, Arg-47, Ile-152, Arg-170 and Thr-244 associated with the disease HP1
Tissue Location Liver.
Research Areas
Citations (0)

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Background

AGXT is expressed only in the liver and protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification.

References

Cellini,B., J. Biol. Chem. 284 (13), 8349-8358 (2009)
Bertoldi,M., Biochim. Biophys. Acta 1784 (9), 1356-1362 (2008)

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$ 295.00
$ 99.00
Cat# AP6500c
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(40 western blots)
Availability: In Stock
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