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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EEF1D Antibody (N-term)   

EEF1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EEF1D Antibody (N-term) AP6523a
    Western blot analysis of EEF1D antibody (N-term) (Cat.# AP6523a) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab.
  • IHC-P - EEF1D Antibody (N-term) AP6523a
    Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - EEF1D Antibody (N-term) AP6523a
    EEF1D Antibody (N-term) (Cat. #AP6523a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29692
Other Accession Q68FR9, P57776, Q4R3D4
Reactivity Human
Predicted Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31122 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 1936
Other Names Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D
Target/Specificity This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1D
Synonyms EF1D
Function Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1- alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
Cellular Location Isoform 2: Nucleus
Tissue Location Isoform 2 is specifically expressed in brain, cerebellum and testis
Research Areas
Citations (0)

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EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.


Yang,S., BMC Cancer 7, 211 (2007)
Mulner-Lorillon,O., J. Biol. Chem. 269 (31), 20201-20207 (1994)

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$ 295.00
$ 99.00
Cat# AP6523a
(40 western blots)
Availability: In Stock
Bulk Size
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