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ALB Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ALB Antibody (C-term) AP6540B
    Anti-ALB Antibody (C-term)at 1:1000 dilution + mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ALB Antibody (C-term) AP6540B
    All lanes : Anti-ALB Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - ALB Antibody (C-term) AP6540B
    Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP6540b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - ALB Antibody (C-term) AP6540B
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP6540b performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - ALB Antibody (C-term) AP6540B
    ALB Antibody (C-term) (Cat. #AP6540b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, FC, E
Primary Accession P02768
Other Accession A2V9Z4, P35747
Reactivity Human, Mouse
Predicted Horse, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 69367 Da
Antigen Region 540-569 aa
Additional Information
Gene ID 213
Other Names Serum albumin, ALB
Target/Specificity This ALB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ALB.
Dilution WB~~1:1000
IHC-P-Leica~~1:500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALB
Function Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Cellular Location Secreted.
Tissue Location Plasma.
Research Areas
Citations ( 0 )

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

References

Liu,Z., Biophys. J. 96 (10), 3917-3925 (2009)
Minchiotti,L., Hum. Mutat. 29 (8), 1007-1016 (2008)

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$ 365.00
$ 140.00
Cat# AP6540B
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