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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   TRAP Antibody (N-term)   

TRAP Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TRAP Antibody (N-term) AP6545A
    All lanes : Anti-TRAP Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TRAP Antibody (N-term) AP6545A
    Western blot analysis of TRAP antibody (N-term) (Cat.# AP6545a) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab.
  • IHC-P - TRAP Antibody (N-term) AP6545A
    TRAP Antibody (N-term) (Cat.# AP6545a) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - TRAP Antibody (N-term) AP6545A
    Confocal immunofluorescent analysis of TRAP Antibody (N-term)(Cat#AP6545a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - TRAP Antibody (N-term) AP6545A
    TRAP antibody (N-term) (Cat.# AP6545a) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29965
Other Accession Q95MQ5, P51749
Reactivity Human
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29274 Da
Antigen Region 33-62 aa
Additional Information
Gene ID 959
Other Names CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP
Target/Specificity This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD40LG
Synonyms CD40L, TNFSF5, TRAP
Function Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Cellular Location Cell membrane; Single-pass type II membrane protein
Tissue Location Specifically expressed on activated CD4+ T- lymphocytes
Research Areas
Citations (0)

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TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.


Volmar,C.H., Exp. Cell Res. 315 (13), 2265-2274 (2009)
Chai,H., Surgery 146 (1), 5-11 (2009)

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$ 295.00
$ 99.00
Cat# AP6545A
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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