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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IGJ Antibody (N-term)   

IGJ Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IGJ Antibody (N-term) AP6548a
    Western blot analysis of IGJ antibody (N-term) (Cat.# AP6548a) in mouse spleen lysates (35ug/lane). IGJ (arrow) was detected using the purified Pab.
  • IHC-P - IGJ Antibody (N-term) AP6548a
    Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - IGJ Antibody (N-term) AP6548a
    IGJ Antibody (N-term) (Cat. #AP6548a) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P01591
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18099 Da
Antigen Region 32-61 aa
Additional Information
Gene ID 3512
Other Names Immunoglobulin J chain, IGJ, IGCJ
Target/Specificity This IGJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human IGJ.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGJ Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGJ
Synonyms IGCJ
Function Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

References

Ramachandran,P., J. Proteome Res. 5 (6), 1493-1503 (2006)
Liu,T., J. Proteome Res. 4 (6), 2070-2080 (2005)

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Cat# AP6548a
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(40 western blots)
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