|Application ||WB, IHC-P, FC, E|
|Calculated MW||124029 Da|
|Antigen Region||681-708 aa|
|Other Names||APC membrane recruitment protein 1, Amer1, Protein FAM123B, Wilms tumor gene on the X chromosome protein, AMER1, FAM123B, WTX|
|Target/Specificity||This WTX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 681-708 amino acids from the Central region of human WTX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WTX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2|
|Tissue Location||Detected in fetal and adult kidney, brain and spleen.|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
WTX is involved in kidney development.
Rivera,M.N., Proc. Natl. Acad. Sci. U.S.A. 106 (20), 8338-8343 (2009) Fukuzawa,R., Oncogene 28 (8), 1063-1075 (2009)
If you have any additional inquiries please email technical services at email@example.com.