|Application ||WB, IHC-P, FC, E|
|Calculated MW||124029 Da|
|Antigen Region||681-708 aa|
|Other Names||APC membrane recruitment protein 1, Amer1, Protein FAM123B, Wilms tumor gene on the X chromosome protein, AMER1, FAM123B, WTX|
|Target/Specificity||This WTX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 681-708 amino acids from the Central region of human WTX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||WTX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2|
|Tissue Location||Detected in fetal and adult kidney, brain and spleen.|
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Provided below are standard protocols that you may find useful for product applications.
WTX is involved in kidney development.
Rivera,M.N., Proc. Natl. Acad. Sci. U.S.A. 106 (20), 8338-8343 (2009) Fukuzawa,R., Oncogene 28 (8), 1063-1075 (2009)
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