|Application ||WB, IHC-P, FC, E|
|Calculated MW||53366 Da|
|Antigen Region||261-290 aa|
|Other Names||Cathepsin F, CATSF, CTSF|
|Target/Specificity||This CTSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the Central region of human CTSF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CTSF Antibody (Center D276) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.|
|Tissue Location||High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus|
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Provided below are standard protocols that you may find useful for product applications.
Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF is a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway.
Kaakinen,R., Atherosclerosis 192 (2), 323-327 (2007)
Oorni,K., J. Biol. Chem. 279 (33), 34776-34784 (2004)
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