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ASPN Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP6604c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP6604c-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Backgrounds
ASPN Antibody (Center) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | Q9BXN1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 43417 Da |
ASPN Antibody (Center) - Additional info | |
| Gene ID 54829 | |
| Other Names ASPN; PLAP1; SLRR1C; Asporin; Periodontal ligament-associated protein 1 | |
| Target/Specificity This ASPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247~277 amino acids from the Center region of human ASPN. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ASPN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ASPN Antibody (Center) - Protein Information | |
| Name ASPN | |
| Synonyms PLAP1, SLRR1C | |
| Function Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF- beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity | |
| Cellular Location Secreted, extracellular space, extracellular matrix | |
| Tissue Location Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Co-localizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage | |
ASPN Antibody (Center) - Related products
AP6604c: ASPN Antibody (Center)
ASPN Antibody (Center) - Application data
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Western blot analysis of ASPN antibody (Center) (Cat. #AP6604c) in HL60 cell line lysates(35ug/lane). ASPN (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with ASPN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of ASPN Antibody (Center) (Cat. #AP6604c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ASPN Antibody (Center) - Research Areas
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BACKGROUND
ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin.
REFERENCES
Gruber,H.E., Arthritis Res. Ther. 11 (2), R47 (2009) Ikegawa,S., Curr. Med. Chem. 15 (7), 724-728 (2008)
